@dominoman2 said in Changing audio output from 3.5mm Headphone to USB Headset, using RetroPie Menus <tag>audio</tag>:
When I run the scripts I have to restart EmulationStation for the sound output changes to take effect
I guess that for EmulationStation to pick up the changes you need to modify the audio settings for the sound to be re-initialized, but for emulators it shouldn't be a problem.