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Got it, thank you!!! :-)
Having gone back to my old pre 5.4 build Retropie I found I needed to downgrade my Kernel due to glitching graphics. Since I have done this I am convinced that Retropie, especially N64 is still running slightly smoother than it did before.
I just created an issue for this at the Raspberry Pi github: https://github.com/raspberrypi/linux/issues/3935
