Can you forgive me little rant earlier? Sorry. I've had a good play with it now and I think it's working. And I understand what you mean by pressing the hotkey and F1. Is there a way to have it by pressing F1 alone?
I've Reset Retroarch config and plugged in a Wired Mouse and keyboard and OP Wolf is working now.
I've also changed my RetoArch to Ozone just like my PC. It's much less confusing now. It's going to take some time to get used to this :)