@mitu said in Usb drive mounting problems:
Just assign a letter in Windows to the partition and erase the folder on it.
So yeah, apparently that was the issue, that somehow a retropie-mount folder was also in the EFI partition:
b69d38b3-7559-4999-a9cb-f8773c110bf9-image.png
Assigning letter H: to the EFI partition and checking through admin in powershell I can see that there is a folder structure and it looks the same as what I see on the desktop on the RPI400 when it fails to load the HDD. I just removed everything from H:
85494f32-f517-47ae-bf02-f7388969a5f6-image.png
So let's see how it behaves from now on. If I don't come back to this thread asume that it was solved.
Thank you very much both of you for your help!