@sebretro said in Periodic stuttering/freezing with RetroPi4.4 on a Raspberry 3B+ in every game (1-2seconds):

Does anyone have know about this problem? Thank you for your help.

Looks like an insufficient power problem - the RPI underclocks itself not having enough power. Take the PI out of the case and do some testing to see if you get the same problems. The NesPI cases are notorious for this kind of problems.