@nicole After writing the Retropie image to the SD card, 2 partitions will be created into the card, the first one (256 MB) containing boot files and configurations for the raspberry pi, the second one, will handle the Linux operating system which Retropie works from within. The Retropie partition is unreadable by Windows.
After booting the raspberry pi with the SD card, Retropie will expand the second partition to ensure the entire available space from the card is ready. This is done only once the first time you turn on the raspberry pi with the card. Follow the steps on the screen.
Please take a look at https://retropie.org.uk/docs/First-Installation/
If you need to recover your SD card as it was before writing the image, please use:
https://www.sdcard.org/downloads/formatter/