To update you all.
I did remove the SD card and deleted everthing on it.
Downloaded the lastest linked version from https://retropie.org.uk and followed again the instruction to flash, install and start the retropie.
I configured the gamepad,
I enabled Wifi settings,
I enabled SSH,
I uploaded via WinSCP some roms
and then started a GBC game and it works.
I don't know, what we did last time different, but it works now.
Hugh thanks to all which helps on this topic.
Kind regards
Bigwerth